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Servicebio Inc phosphate buffer solution
Phosphate Buffer Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphate+buffer+solution/buffered+phosphate+saline/pm42321244-331-0-15
Average 86 stars, based on 1 article reviews
phosphate buffer solution - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Incubation:

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x10 5 cells/mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x10 5 /cm 2 ) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x105 cells/ mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x105/cm2) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Cell Culture:

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x10 5 cells/mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x10 5 /cm 2 ) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x105 cells/ mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x105/cm2) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Fluorescence:

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x10 5 cells/mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x10 5 /cm 2 ) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x105 cells/ mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x105/cm2) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Microscopy:

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x10 5 cells/mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x10 5 /cm 2 ) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Article Title: Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Article Snippet: Chondrocytes in good growth status were pre-inoculated into 6-well plates, and the cells were treated as described in 2.7, and assayed using the Calcein-AM/PI Live/Dead Cell Double Staining Kit (Servicebio, China). .. Calcein-AM reagent, PI reagent, and assay buffer were returned to room temperature, cells were digested with 25% trypsin (Servicebio, China) and collected, centrifuged at 1000 rpm for 5 min, the supernatant was removed and washed 2 times using phosphate buffer solution (PBS, Servicebio, China), and Calcein AM/PI assay was added The cells were resuspended (1x105 cells/ mL) in working solution and incubated in a cell culture incubator for 30 min, and finally observed and photographed using a fluorescence microscope (FV31S-SW, Olympus, Japan). .. Chondrocytes were inoculated in 6-well plates (1x105/cm2) and cells were treated as described in 2.7 and assayed using the JC-1 Mitochondrial Membrane Potential Assay Kit (Servicebio, China).

Centrifugation:

Article Title: Identification and Functional Validation of PTH2R as a Therapeutic Target in Lung Adenocarcinoma
Article Snippet: .. After treatment, the cells were collected by centrifugation at 500 rpm for five minutes and washed twice with 1× phosphate buffer solution (PBS, Servicebio, China). .. The cells were reconstituted in 125 μL of 1× binding buffer supplemented with 1.25 μL each of APC-labeled Annexin V and DAPI (Elabscience, Wuhan, China)) added.

Article Title: Identification and Functional Validation of PTH2R as a Therapeutic Target in Lung Adenocarcinoma.
Article Snippet: .. After treatment, the cells were collected by centrifugation at 500 rpm for five minutes and washed twice with 1× phosphate buffer solution (PBS, Servicebio, China). .. The cells were reconstituted in 125 μL of 1× binding buffer supplemented with 1.25 μL each of APC-labeled Annexin V and DAPI (Elabscience, Wuhan, China)) added.

Article Title: Effect of rubusoside, a natural sucrose substitute, on the metabolism of Scardovia wiggsiae: a novel potential cariogenic pathogen.
Article Snippet: Then, successively perform primary staining with crystal violet for 1 min, wash with water, and perform mordant staining with iodine solution for 1 min. After washing with water, decolorize with 95% alcohol by shaking the slide for 20–60 s. Wash with water again and perform counterstaining with acid fuchsin for 1 min. After washing and drying, observe the stained bacterial morphology under an oil immersion microscope (OLYMPUS CX23, China). .. Phosphate buffer solution (PBS, Servicebio, China) was used to wash the bacteria after they were extracted by centrifugation (8000 × g, 10 min, 4 °C). ..

Article Title: Effect of rubusoside, a natural sucrose substitute, on the metabolism of Scardovia wiggsiae : a novel potential cariogenic pathogen
Article Snippet: Then, successively perform primary staining with crystal violet for 1 min, wash with water, and perform mordant staining with iodine solution for 1 min. After washing with water, decolorize with 95% alcohol by shaking the slide for 20–60 s. Wash with water again and perform counterstaining with acid fuchsin for 1 min. After washing and drying, observe the stained bacterial morphology under an oil immersion microscope (OLYMPUS CX23, China). .. Phosphate buffer solution (PBS, Servicebio, China) was used to wash the bacteria after they were extracted by centrifugation (8000 × g , 10 min, 4 °C). ..

Bacteria:

Article Title: Effect of rubusoside, a natural sucrose substitute, on the metabolism of Scardovia wiggsiae: a novel potential cariogenic pathogen.
Article Snippet: Then, successively perform primary staining with crystal violet for 1 min, wash with water, and perform mordant staining with iodine solution for 1 min. After washing with water, decolorize with 95% alcohol by shaking the slide for 20–60 s. Wash with water again and perform counterstaining with acid fuchsin for 1 min. After washing and drying, observe the stained bacterial morphology under an oil immersion microscope (OLYMPUS CX23, China). .. Phosphate buffer solution (PBS, Servicebio, China) was used to wash the bacteria after they were extracted by centrifugation (8000 × g, 10 min, 4 °C). ..

Article Title: Effect of rubusoside, a natural sucrose substitute, on the metabolism of Scardovia wiggsiae : a novel potential cariogenic pathogen
Article Snippet: Then, successively perform primary staining with crystal violet for 1 min, wash with water, and perform mordant staining with iodine solution for 1 min. After washing with water, decolorize with 95% alcohol by shaking the slide for 20–60 s. Wash with water again and perform counterstaining with acid fuchsin for 1 min. After washing and drying, observe the stained bacterial morphology under an oil immersion microscope (OLYMPUS CX23, China). .. Phosphate buffer solution (PBS, Servicebio, China) was used to wash the bacteria after they were extracted by centrifugation (8000 × g , 10 min, 4 °C). ..

other:

Article Title: Neuron-targeting piezoelectric microneedles disrupt pro-tumorigenic neuron-immune crosstalk and restore anti-tumor immunity in melanoma.
Article Snippet: Phosphate buffer solution (PBS, catalogue number G4202) and DMEM/F12 (catalogue number G4610) were purchased from Servicebio.



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